Proteomic profile evolution during steatosis development in ducks.

TitleProteomic profile evolution during steatosis development in ducks.
Publication TypeJournal Article
Year of Publication2012
AuthorsBax, ML, Chambon, C, Marty-Gasset, N, Rémignon, H, Fernandez, X, Molette, C
JournalPoult Sci
Date Published2012 Jan
KeywordsAnimals, Antioxidants, Avian Proteins, Calcium, Carrier Proteins, Chromatography, Liquid, Ducks, Electrophoresis, Gel, Two-Dimensional, Fatty Liver, Gene Expression Profiling, Liver, Male, Poultry Diseases, Protein Biosynthesis, Proteome, Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

We investigated a protein profile evolution during steatosis in ducks using 2-dimensional electrophoresis gels to better understand the mechanisms underlying liver steatosis at the level of hepatic proteins in waterfowl. Two-dimensional electrophoresis gels were performed in the liver at different stages of steatosis in the duck. Mule ducks were slaughtered after 0, 14, or 23 meals of overfeeding, according to commercial conditions. Thirty-one proteic spots were differentially expressed between 3 or 2 durations of overfeeding: 3 spots were differentially expressed between the 3 times and 28 spots were differentially expressed between 2 times. The identified proteins (14) could be regrouped into 5 categories: enzymes, translation factors, proteins involved in cell structure, proteins with antioxidant properties, and proteins that can link calcium. This study opens new research areas in the understanding of steatosis in waterfowl, such as cell structure and oxidative stress.

Alternate JournalPoult. Sci.
PubMed ID22184436