Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.

TitleGenetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.
Publication TypeJournal Article
Year of Publication2012
AuthorsBonnefont, CMD, Rainard, P, Cunha, P, Gilbert, FB, Toufeer, M, Aurel, M-R, Rupp, R, Foucras, G
JournalPhysiol Genomics
Volume44
Issue7
Pagination403-16
Date Published2012 Apr 2
ISSN1531-2267
KeywordsAnimals, Cluster Analysis, Epithelial Cells, Female, Gene Expression Profiling, Gene Regulatory Networks, Genetic Predisposition to Disease, Mammary Glands, Animal, Mastitis, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Sheep, Staphylococcus aureus, Subcellular Fractions
Abstract

Staphylococcus aureus is a prevalent pathogen for mastitis in dairy ruminants and is responsible for both clinical and subclinical mastitis. Mammary epithelial cells (MEC) represent not only a physical barrier against bacterial invasion but are also active players of the innate immune response permitting infection clearance. To decipher their functions in general and in animals showing different levels of genetic predisposition to Staphylococcus in particular, MEC from ewes undergoing a divergent selection on milk somatic cell count were stimulated by S. aureus. MEC response was also studied according to the stimulation condition with live bacteria or culture supernatant. The early MEC response was studied during a 5 h time course by microarray to identify differentially expressed genes with regard to the host genetic background and as a function of the conditions of stimulation. In both conditions of stimulation, metabolic processes were altered, the apoptosis-associated pathways were considerably modified, and inflammatory and immune responses were enhanced with the upregulation of il1a, il1b, and tnfa and several chemokines known to enhance neutrophil (cxcl8) or mononuclear leukocyte (ccl20) recruitment. Genes associated with oxidative stress were increased after live bacteria stimulation, whereas immune response-related genes were higher after supernatant stimulation in the early phase. Only 20 genes were differentially expressed between Staphylococcus spp-mastitis resistant and susceptible animals without any clearly defined role on the control of infection. To conclude, this suggests that MEC may not represent the cell type at the origin of the difference of mastitis susceptibility, at least as demonstrated in our genetic model. Supernatant or heat-killed S. aureus produce biological effects that are essentially different from those induced by live bacteria.

URLhttp://physiolgenomics.physiology.org/content/44/7/403.full
DOI10.1152/physiolgenomics.00155.2011
Alternate JournalPhysiol. Genomics
PubMed ID22337903