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Two-Dimensional Zymography of Proteases from Steatotic Duck Liver.

TitleTwo-Dimensional Zymography of Proteases from Steatotic Duck Liver.
Publication TypeJournal Article
Year of Publication2017
AuthorsWilkesman, J, Padrón, MFernanda, Kurz, L, Rémignon, H
JournalMethods Mol Biol
Volume1626
Pagination157-166
Date Published2017
ISSN1940-6029
KeywordsAnimals, Cathepsins, Ducks, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Enzyme Assays, Fatty Liver, Liver, Metalloendopeptidases, Peptide Hydrolases
Abstract

Protease activity present in liver cells with steatosis can be electrophoretically characterized. Zymographic techniques allow semi-quantitative results, successfully detecting cathepsin and metalloprotease activity using polyacrylamide gels copolymerized with gelatin and quantified by densitometry. By using specific inhibitors, the identity of the proteases can be confirmed. 2D zymography allows the determination of both Mand pI of the metalloprotease and cathepsin activity present in the homogenates. The analysis of liver proteases activities in force fed ducks may elucidate the mechanisms behind steatosis development.

DOI10.1007/978-1-4939-7111-4_14
Alternate JournalMethods Mol. Biol.
PubMed ID28608208