Whole-genome resequencing of honeybee drones to detect genomic selection in a population managed for royal jelly.

TitleWhole-genome resequencing of honeybee drones to detect genomic selection in a population managed for royal jelly.
Publication TypeJournal Article
Year of Publication2016
AuthorsWragg, D, Marti-Marimon, M, Basso, B, Bidanel, J-P, Labarthe, E, Bouchez, O, Le Conte, Y, Vignal, A
JournalSci Rep
Volume6
Pagination27168
Date Published2016
ISSN2045-2322
Abstract

Four main evolutionary lineages of A. mellifera have been described including eastern Europe (C) and western and northern Europe (M). Many apiculturists prefer bees from the C lineage due to their docility and high productivity. In France, the routine importation of bees from the C lineage has resulted in the widespread admixture of bees from the M lineage. The haplodiploid nature of the honeybee Apis mellifera, and its small genome size, permits affordable and extensive genomics studies. As a pilot study of a larger project to characterise French honeybee populations, we sequenced 60 drones sampled from two commercial populations managed for the production of honey and royal jelly. Results indicate a C lineage origin, whilst mitochondrial analysis suggests two drones originated from the O lineage. Analysis of heterozygous SNPs identified potential copy number variants near to genes encoding odorant binding proteins and several cytochrome P450 genes. Signatures of selection were detected using the hapFLK haplotype-based method, revealing several regions under putative selection for royal jelly production. The framework developed during this study will be applied to a broader sampling regime, allowing the genetic diversity of French honeybees to be characterised in detail.

DOI10.1038/srep27168
Alternate JournalSci Rep
PubMed ID27255426
PubMed Central IDPMC4891733