A gene-based radiation hybrid map of chicken microchromosome 14: comparison to human and alignment to the assembled chicken sequence.

TitleA gene-based radiation hybrid map of chicken microchromosome 14: comparison to human and alignment to the assembled chicken sequence.
Publication TypeJournal Article
Year of Publication2005
AuthorsMorisson, M, Leroux, S, Jiguet-Jiglaire, C, Assaf, S, Pitel, F, Lagarrigue, S, Bardes, S, Feve, K, Faraut, T, Milan, D, Vignal, A
JournalGenet Sel Evol
Volume37
Issue2
Pagination229-51
Date Published2005 Mar-Apr
ISSN0999-193X
KeywordsAnimals, Base Sequence, Chickens, Chromosomes, Computational Biology, DNA Primers, Expressed Sequence Tags, Gene Order, Genetic Markers, Humans, Microsatellite Repeats, Molecular Sequence Data, Radiation Hybrid Mapping, Sequence Alignment, Sequence Analysis, DNA
Abstract

We present a gene-based RH map of the chicken microchromosome GGA14, known to have synteny conservations with human chromosomal regions HSA16p13.3 and HSA17p11.2. Microsatellite markers from the genetic map were used to check the validity of the RH map and additional markers were developed from chicken EST data to yield comparative mapping data. A high rate of intra-chromosomal rearrangements was detected by comparison to the assembled human sequence. Finally, the alignment of the RH map to the assembled chicken sequence showed a small number of discordances, most of which involved the same region of the chromosome spanning between 40.5 and 75.9 cR(6000) on the RH map.

DOI10.1051/gse:2004046
Alternate JournalGenet. Sel. Evol.
PubMed ID16194526
PubMed Central IDPMC2697232