Epilepsy caused by an abnormal alternative splicing with dosage effect of the SV2A gene in a chicken model.

TitleEpilepsy caused by an abnormal alternative splicing with dosage effect of the SV2A gene in a chicken model.
Publication TypeJournal Article
Year of Publication2011
AuthorsDouaud, M, Feve, K, Pituello, F, Gourichon, D, Boitard, S, Leguern, E, Coquerelle, G, Vieaud, A, Batini, C, Naquet, R, Vignal, A, Tixier-Boichard, M, Pitel, F
JournalPLoS One
Volume6
Issue10
Paginatione26932
Date Published2011
ISSN1932-6203
KeywordsAlternative Splicing, Animals, Chickens, Disease Models, Animal, Epilepsy, Gene Dosage, Humans, Membrane Glycoproteins, Mice, Mice, Knockout, Mutation, Nerve Tissue Proteins, Phylogeny, Seizures
Abstract

Photosensitive reflex epilepsy is caused by the combination of an individual's enhanced sensitivity with relevant light stimuli, such as stroboscopic lights or video games. This is the most common reflex epilepsy in humans; it is characterized by the photoparoxysmal response, which is an abnormal electroencephalographic reaction, and seizures triggered by intermittent light stimulation. Here, by using genetic mapping, sequencing and functional analyses, we report that a mutation in the acceptor site of the second intron of SV2A (the gene encoding synaptic vesicle glycoprotein 2A) is causing photosensitive reflex epilepsy in a unique vertebrate model, the Fepi chicken strain, a spontaneous model where the neurological disorder is inherited as an autosomal recessive mutation. This mutation causes an aberrant splicing event and significantly reduces the level of SV2A mRNA in homozygous carriers. Levetiracetam, a second generation antiepileptic drug, is known to bind SV2A, and SV2A knock-out mice develop seizures soon after birth and usually die within three weeks. The Fepi chicken survives to adulthood and responds to levetiracetam, suggesting that the low-level expression of SV2A in these animals is sufficient to allow survival, but does not protect against seizures. Thus, the Fepi chicken model shows that the role of the SV2A pathway in the brain is conserved between birds and mammals, in spite of a large phylogenetic distance. The Fepi model appears particularly useful for further studies of physiopathology of reflex epilepsy, in comparison with induced models of epilepsy in rodents. Consequently, SV2A is a very attractive candidate gene for analysis in the context of both mono- and polygenic generalized epilepsies in humans.

DOI10.1371/journal.pone.0026932
Alternate JournalPLoS ONE
PubMed ID22046416
PubMed Central IDPMC3203167