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New investigations around CYP11A1 and its possible involvement in an androstenone QTL characterised in Large White pigs.

TitleNew investigations around CYP11A1 and its possible involvement in an androstenone QTL characterised in Large White pigs.
Publication TypeJournal Article
Year of Publication2011
AuthorsRobic, A, Le Mignon, G, Feve, K, Larzul, C, Riquet, J
JournalGenet Sel Evol
Date Published2011
KeywordsAdipose Tissue, Androsterone, Animals, Cholesterol Side-Chain Cleavage Enzyme, Genome-Wide Association Study, Male, Polymorphism, Single Nucleotide, Quantitative Trait Loci, Sus scrofa, Testis

BACKGROUND: Previously, in boars with extreme androstenone levels, differential expression of the CYP11A1 gene in the testes has been characterised. CYP11A1 is located in a region where a QTL influencing boar fat androstenone levels has been detected in a Large White pig population. Clarifying the role of CYP11A1 in boar taint is important because it catalyses the initial step of androstenone synthesis and also of steroid synthesis.RESULTS: A genome-wide association study located CYP11A1 at approximately 1300 kb upstream from SNP H3GA0021967, defining the centre of the region containing the QTL for androstenone variation. In this study, we partially sequenced the CYP11A1 gene and identified several new single nucleotide polymorphisms (SNP) within it. Characterisation of one animal, heterozygous for CYP11A1 testicular expression but homozygous for a haplotype of a large region containing CYP11A1, revealed that variation of CYP11A1 expression is probably regulated by a mutation located downstream from the SNP H3GA0021967. We analysed CYP11A1 expression in LW families according to haplotypes of the QTL region's centre. Effects of haplotypes on CYP11A1 expression and on androstenone accumulation were not concordant.CONCLUSION: This study shows that testicular expression of CYP11A1 is not solely responsible for the QTL influencing boar fat androstenone levels. As a conclusion, we propose to refute the hypothesis that a single mutation located near the centre of the QTL region could control androstenone accumulation in fat by regulating the CYP11A1 expression.

Alternate JournalGenet. Sel. Evol.
PubMed ID21504607
PubMed Central IDPMC3098772