The carnitine acetyltransferase gene (CRAT): a characterization of porcine transcripts with insights into the 5'-end variants of mammalian transcripts and their possible sub-cellular localization.

TitleThe carnitine acetyltransferase gene (CRAT): a characterization of porcine transcripts with insights into the 5'-end variants of mammalian transcripts and their possible sub-cellular localization.
Publication TypeJournal Article
Year of Publication2009
AuthorsRobic, A, Faraut, T, Liaubet, L, Milan, D
JournalCell Mol Biol Lett
Volume14
Issue1
Pagination90-9
Date Published2009
ISSN1689-1392
Keywords5' Untranslated Regions, Amino Acid Sequence, Animals, Carnitine O-Acetyltransferase, Gene Expression Regulation, Enzymologic, Humans, Mammals, Molecular Sequence Data, Protein Transport, RNA, Messenger, Subcellular Fractions, Swine, Transcription, Genetic
Abstract

Carnitine acetyltransferase (CRAT) is an important enzyme for energy homeostasis and fat metabolism. We characterized the predicted full length cDNA sequence of the porcine CRAT gene. Its structure is very similar to that in humans with respect to the size and organization of the 14 exons. We demonstrated the existence of a porcine alternative transcript resulting from a partial intron-retention at the 5' end of exon 2. To perform a comparison of the 5' end variants of the mammalian CRAT gene, we analyzed the Genbank data, and here we propose a new 5' variant for dog, rat and mouse. In contrast to other mammals where this variant encodes a shorter protein (-21 aa in human, mouse and rat, and -14 aa in dog), the pig variant encodes for a longer protein (+18 aa). In all mammalian species, variant 1 has a high probability of a preferential mitochondrial sub-cellular localization. Nevertheless, it is not evident, in particular in porcine and dog species, that the second variant is associated with a different sub-cellular specificity.

DOI10.2478/s11658-008-0036-3
Alternate JournalCell. Mol. Biol. Lett.
PubMed ID18839069