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Early Inoculation of Microbial Suspension in Suckling Piglets Affects the Transmission of Maternal Microbiota and the Associated Antibiotic Resistance Genes.

TitleEarly Inoculation of Microbial Suspension in Suckling Piglets Affects the Transmission of Maternal Microbiota and the Associated Antibiotic Resistance Genes.
Publication TypeJournal Article
Year of Publication2020
AuthorsAchard, CS, Dupouy, V, Cauquil, L, Arpaillange, N, Bousquet-Melou, A, Le Floc'h, N, Zemb, O
JournalMicroorganisms
Volume8
Issue10
Date Published2020 Oct 13
ISSN2076-2607
Abstract

Antibiotic resistance of microbes thriving in the animal gut is a growing concern for public health as it may serve as a hidden reservoir for antibiotic resistance genes (ARGs). We compared 16 control piglets to 24 piglets fed for 3 weeks with S1 or S2 fecal suspensions from two sows that were not exposed to antibiotics for at least 6 months: the first suspension decreased the erythromycin resistance gene and the aminoglycoside phosphotransferase gene conferring resistance to kanamycine , while the second decreased the tetracycline resistance gene , with an unexpected increase in ARGs. Using 16S RNA sequencing, we identified microbial species that are likely to carry ARGs, such as the lincosamide nucleotidyltransferase , the cephalosporinase , and the tetracycline resistance genes and , as well as microbes that never co-exist with the tetracycline resistance gene , the erythromycin resistance gene and . Since 73% of the microbes detected in the sows were not detected in the piglets at weaning, a neutral model was applied to estimate whether a microbial species is more important than chance would predict. This model confirmed that force-feeding modifies the dynamics of gut colonization. In conclusion, early inoculation of gut microbes is an interesting possibility to stimulate gut microbiota towards a desirable state in pig production, but more work is needed to be able to predict which communities should be used.

DOI10.3390/microorganisms8101576
Alternate JournalMicroorganisms
PubMed ID33066283
Grant Listmicroreset / / Institut Carnot Santé Animale /