Analysis of Meiotic Segregation Pattern and Interchromosomal Effects in a Bull Heterozygous for a 3/16 Robertsonian Translocation.

TitleAnalysis of Meiotic Segregation Pattern and Interchromosomal Effects in a Bull Heterozygous for a 3/16 Robertsonian Translocation.
Publication TypeJournal Article
Year of Publication2018
AuthorsBarasc, H, Mouney-Bonnet, N, Peigney, C, Calgaro, A, Revel, C, Mary, N, Ducos, A, Pinton, A
JournalCytogenet Genome Res
Volume156
Issue4
Pagination197-203
Date Published2018
ISSN1424-859X
KeywordsAnimals, Cattle, Chromosome Segregation, Chromosomes, Mammalian, Cytogenetic Analysis, In Situ Hybridization, Fluorescence, Male, Meiosis, Spermatozoa, Translocation, Genetic
Abstract

Robertsonian translocations are the most frequent chromosomal rearrangements detected in cattle. Here, we report on the detection of a new Robertsonian translocation between chromosomes BTA3 and BTA16. This rob(3;16) was dicentric, suggesting that its occurrence was recent. FISH analysis of decondensed sperm nuclei revealed a relatively low rate of unbalanced gametes produced by adjacent segregation (5.87%). In addition, and for the first time in bovines, a significant interchromosomal effect (ICE) was detected for 2 different autosomes: BTA17 (global disomy + nullisomy rate of 9%) and BTA20 (1.8%). These results suggest that ICE should be taken into consideration when assessing the putative effect of Robertsonian translocations on reproduction.

DOI10.1159/000494289
Alternate JournalCytogenet. Genome Res.
PubMed ID30504703