Title | Establishment of ruminal bacterial community in dairy calves from birth to weaning is sequential. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Rey, M, Enjalbert, F, Combes, S, Cauquil, L, Bouchez, O, Monteils, V |
Journal | J Appl Microbiol |
Volume | 116 |
Issue | 2 |
Pagination | 245-57 |
Date Published | 2014 Feb |
ISSN | 1365-2672 |
Keywords | Animals, Animals, Newborn, Animals, Suckling, Bacteria, Bacteroides, Bacteroidetes, Cattle, Colostrum, Diet, DNA, Bacterial, DNA, Ribosomal, Fatty Acids, Volatile, Female, Fusobacterium, Male, Pasteurellaceae, Prevotella, Proteobacteria, RNA, Bacterial, RNA, Ribosomal, 16S, Rumen, Streptococcus, Weaning |
Abstract | AIM: Establishment of ruminal bacterial community in dairy calves.METHODS AND RESULTS: Rumen bacterial community was analysed on 6 calves bred according to commercial practices from day one to weaning at day 83 of age, using 454 16S rRNA-based pyrosequencing. Samples taken at day 1 did not produce amplicons. Analysis of data revealed a three-stage implantation process with a progressive but important shift of composition. At day 2, the bacterial community was mainly composed of Proteobacteria (70%) and Bacteroidetes (14%), and Pasteurellaceae was the dominant family (58%). The bacterial community abruptly changed between days 2 and 3, and until day 12, dominant genera were Bacteroides (21%), Prevotella (11%), Fusobacterium (5%) and Streptococcus (4%). From 15 to 83 days, when solid food intake rapidly increased, Prevotella became dominant (42%) and many genera strongly decreased or were no longer detected. A limited number of bacteria genera correlated with feed intake, rumen volatile fatty acids and enzymatic activities.CONCLUSION: The ruminal bacterial community is established before intake of solid food, but solid food arrival in turn shapes this community.SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides insight into the establishment of calves' rumen bacterial community and suggests a strong effect of diet. |
DOI | 10.1111/jam.12405 |
Alternate Journal | J. Appl. Microbiol. |
PubMed ID | 24279326 |
Establishment of ruminal bacterial community in dairy calves from birth to weaning is sequential.
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