Meiotic pairing and gene expression disturbance in germ cells from an infertile boar with a balanced reciprocal autosome-autosome translocation.

TitleMeiotic pairing and gene expression disturbance in germ cells from an infertile boar with a balanced reciprocal autosome-autosome translocation.
Publication TypeJournal Article
Year of Publication2016
AuthorsBarasc, H, Congras, A, Mary, N, Trouilh, L, Marquet, V, Ferchaud, S, Raymond-Letron, I, Calgaro, A, Loustau-Dudez, A-M, Mouney-Bonnet, N, Acloque, H, Ducos, A, Pinton, A
JournalChromosome Res
Volume24
Issue4
Pagination511-527
Date Published2016 Dec
ISSN1573-6849
Abstract

Individuals carrying balanced constitutional reciprocal translocations generally have a normal phenotype, but often present reproductive disorders. The aim of our research was to analyze the meiotic process in an oligoasthenoteratospermic boar carrying an asymmetric reciprocal translocation involving chromosomes 1 and 14. Different multivalent structures (quadrivalent and trivalent plus univalent) were identified during chromosome pairing analysis. Some of these multivalents were characterized by the presence of unpaired autosomal segments with histone γH2AX accumulation sometimes associated with the XY body. Gene expression in spermatocytes was studied by RNA-DNA-FISH and microarray-based testis transcriptome analysis. Our results revealed a decrease in gene expression for chromosomes 1 and 14 and an up-regulated expression of X-chromosome genes for the translocated boar compared with normal individuals. We hypothesized that the observed meiotic arrest and reproductive failure in this boar might be due to silencing of crucial autosomal genes (MSUC) and disturbance of meiotic sex chromosome inactivation (MSCI). Further analysis revealed abnormal meiotic recombination (frequency and distribution) and the production of a high rate of unbalanced spermatozoa.

DOI10.1007/s10577-016-9533-9
Alternate JournalChromosome Res.
PubMed ID27484982
PubMed Central IDPMC5167775