|Title||A combinatorial code for CPE-mediated translational control.|
|Publication Type||Journal Article|
|Year of Publication||2008|
|Authors||Piqué, M, López, JManuel, Foissac, S, Guigó, R, Méndez, R|
|Date Published||2008 Feb 08|
|Keywords||3' Untranslated Regions, Animals, Cyclin B, Cytoplasm, Gene Expression Regulation, Humans, Meiosis, Mice, mRNA Cleavage and Polyadenylation Factors, Mutagenesis, Oocytes, Polyadenylation, Progesterone, Protein Biosynthesis, RNA 3' Polyadenylation Signals, RNA, Messenger, Stored, RNA-Binding Proteins, Transcription Factors, Xenopus laevis, Xenopus Proteins|
Cytoplasmic polyadenylation plays a key role in the translational control of mRNAs driving biological processes such as gametogenesis, cell-cycle progression, and synaptic plasticity. What determines the distinct time of polyadenylation and extent of translational control of a given mRNA, however, is poorly understood. The polyadenylation-regulated translation is controlled by the cytoplasmic polyadenylation element (CPE) and its binding protein, CPEB, which can assemble both translational repression or activation complexes. Using a combination of mutagenesis and experimental validation of genome-wide computational predictions, we show that the number and relative position of two elements, the CPE and the Pumilio-binding element, with respect to the polyadenylation signal define a combinatorial code that determines whether an mRNA will be translationally repressed by CPEB, as well as the extent and time of cytoplasmic polyadenylation-dependent translational activation.