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Modeling of autosomal-dominant retinitis pigmentosa in Caenorhabditis elegans uncovers a nexus between global impaired functioning of certain splicing factors and cell type-specific apoptosis.

TitleModeling of autosomal-dominant retinitis pigmentosa in Caenorhabditis elegans uncovers a nexus between global impaired functioning of certain splicing factors and cell type-specific apoptosis.
Publication TypeJournal Article
Year of Publication2015
AuthorsRubio-Peña, K, Fontrodona, L, Aristizábal-Corrales, D, Torres, S, Cornes, E, García-Rodríguez, FJ, Serrat, X, González-Knowles, D, Foissac, S, Porta-De-La-Riva, M, Cerón, J
JournalRNA
Volume21
Issue12
Pagination2119-31
Date Published2015 Dec
ISSN1469-9001
KeywordsAnimals, Apoptosis, Ataxia Telangiectasia Mutated Proteins, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Genes, Dominant, Organ Specificity, Repressor Proteins, Retinitis Pigmentosa, Ribonucleoprotein, U4-U6 Small Nuclear, Ribonucleoprotein, U5 Small Nuclear, RNA Interference, RNA Splicing
Abstract

Retinitis pigmentosa (RP) is a rare genetic disease that causes gradual blindness through retinal degeneration. Intriguingly, seven of the 24 genes identified as responsible for the autosomal-dominant form (adRP) are ubiquitous spliceosome components whose impairment causes disease only in the retina. The fact that these proteins are essential in all organisms hampers genetic, genomic, and physiological studies, but we addressed these difficulties by using RNAi in Caenorhabditis elegans. Our study of worm phenotypes produced by RNAi of splicing-related adRP (s-adRP) genes functionally distinguishes between components of U4 and U5 snRNP complexes, because knockdown of U5 proteins produces a stronger phenotype. RNA-seq analyses of worms where s-adRP genes were partially inactivated by RNAi, revealed mild intron retention in developing animals but not in adults, suggesting a positive correlation between intron retention and transcriptional activity. Interestingly, RNAi of s-adRP genes produces an increase in the expression of atl-1 (homolog of human ATR), which is normally activated in response to replicative stress and certain DNA-damaging agents. The up-regulation of atl-1 correlates with the ectopic expression of the pro-apoptotic gene egl-1 and apoptosis in hypodermal cells, which produce the cuticle, but not in other cell types. Our model in C. elegans resembles s-adRP in two aspects: The phenotype caused by global knockdown of s-adRP genes is cell type-specific and associated with high transcriptional activity. Finally, along with a reduced production of mature transcripts, we propose a model in which the retina-specific cell death in s-adRP patients can be induced through genomic instability.

DOI10.1261/rna.053397.115
Alternate JournalRNA
PubMed ID26490224
PubMed Central IDPMC4647465
Grant ListP40 OD010440 / OD / NIH HHS / United States