A high-resolution radiation hybrid map of chicken chromosome 5 and comparison with human chromosomes.

TitleA high-resolution radiation hybrid map of chicken chromosome 5 and comparison with human chromosomes.
Publication TypeJournal Article
Year of Publication2004
AuthorsPitel, F, Abasht, B, Morisson, M, Crooijmans, RPMA, Plisson-Petit, F, Leroux, S, Feve, K, Bardes, S, Milan, D, Lagarrigue, S, Groenen, MAM, Douaire, M, Vignal, A
JournalBMC Genomics
Date Published2004 Sep 15
KeywordsAnimals, Chickens, Chromosomes, Chromosomes, Human, DNA, Expressed Sequence Tags, Genetic Markers, Humans, Mice, Radiation Hybrid Mapping, Sequence Alignment

BACKGROUND: The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. Moreover, once framework RH maps of a genome have been constructed, a quick location of markers by simple PCR on the RH panel is possible. The chicken ChickRH6 panel recently produced was used here to construct a high resolution RH map of chicken GGA5. To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5.RESULTS: A total of 169 markers (21 microsatellites and 148 ESTs) were typed on the ChickRH6 RH panel, of which 134 were assigned to GGA5. The final map is composed of 73 framework markers extending over a 1315.6 cR distance. The remaining 61 markers were placed alongside the framework markers within confidence intervals.CONCLUSION: The high resolution framework map obtained in this study has markers covering the entire chicken chromosome 5 and reveals the existence of a high number of rearrangements when compared to the human genome. Only two discrepancies were observed in relation to the sequence assembly recently reported for this chromosome.

Alternate JournalBMC Genomics
PubMed ID15369602
PubMed Central IDPMC521070