|Title||Female-specific DNA sequences in the chicken genome.|
|Publication Type||Journal Article|
|Year of Publication||2007|
|Authors||Granevitze, Z, Blum, S, Cheng, H, Vignal, A, Morisson, M, Ben-Ari, G, David, L, Feldman, MWilliam, Weigend, S, Hillel, J|
|Date Published||2007 May-Jun|
|Keywords||Animals, Chickens, Chromosome Mapping, DNA, Female, Genome, Hybrid Cells, Polymerase Chain Reaction, Polymorphism, Single Nucleotide|
Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.
|Alternate Journal||J. Hered.|