Title | Mapping and genotypic analysis of the NK-lysin gene in chicken. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Lee, MOk, Yang, E, Morisson, M, Vignal, A, Huang, Y-Z, Cheng, HH, Muir, WM, Lamont, SJ, Lillehoj, HSoon, Lee, SHyen, Womack, JE |
Journal | Genet Sel Evol |
Volume | 46 |
Pagination | 43 |
Date Published | 2014 |
ISSN | 1297-9686 |
Keywords | Alleles, Animals, Avian Proteins, Breeding, Carboxypeptidases, Chickens, Chromosome Mapping, Chromosomes, DNA Polymerase III, Gene Frequency, Genetic Markers, Genome, Genotype, Phenotype, Polymorphism, Single Nucleotide, Proteolipids, Repressor Proteins, Sequence Analysis, DNA |
Abstract | BACKGROUND: Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds.RESULTS: A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines.CONCLUSIONS: The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes, AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds. |
DOI | 10.1186/1297-9686-46-43 |
Alternate Journal | Genet. Sel. Evol. |
PubMed ID | 25001618 |
PubMed Central ID | PMC4120735 |
Mapping and genotypic analysis of the NK-lysin gene in chicken.
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