Mapping and genotypic analysis of the NK-lysin gene in chicken.

TitleMapping and genotypic analysis of the NK-lysin gene in chicken.
Publication TypeJournal Article
Year of Publication2014
AuthorsLee, MOk, Yang, E, Morisson, M, Vignal, A, Huang, Y-Z, Cheng, HH, Muir, WM, Lamont, SJ, Lillehoj, HSoon, Lee, SHyen, Womack, JE
JournalGenet Sel Evol
Volume46
Pagination43
Date Published2014
ISSN1297-9686
KeywordsAlleles, Animals, Avian Proteins, Breeding, Carboxypeptidases, Chickens, Chromosome Mapping, Chromosomes, DNA Polymerase III, Gene Frequency, Genetic Markers, Genome, Genotype, Phenotype, Polymorphism, Single Nucleotide, Proteolipids, Repressor Proteins, Sequence Analysis, DNA
Abstract

BACKGROUND: Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds.RESULTS: A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines.CONCLUSIONS: The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes, AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.

DOI10.1186/1297-9686-46-43
Alternate JournalGenet. Sel. Evol.
PubMed ID25001618
PubMed Central IDPMC4120735
genorobust